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KMID : 0380020130280050282
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 5 p.282 ~ p.286
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Bioethanol Production from Seaweed Gelidium amansii for Separated Hydrolysis and Fermentation (SHF)
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Ra Chae-Hun
Lee Hyeon-Jun
Shin Myung-Kyo
Kim Sung-Koo
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Abstract
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The seaweed, Gelidium amansii, was fermented to produce bioethanol. Optimal pretreatment condition was determined as 94 mM H2SO4 and 8% (w/v) seaweed slurry at 121oC for 60 min. The mono sugars of 40.4 g/L with 67% of conversion from total carbohydrate of 60.6 g/L with 80 g dw/L G. amansii slurry were obtained by thermal acid hydrolysis pretreatment and enzymatic saccharification. G. amansii hydrolysate was used as the substrate for ethanol production by Kluyveromyces marxianus KCTC 7150 and Candida tropicalis KCTC 7212 using 5L fermentor. The ethanol productions by K. marxianus KCTC 7150 and C. tropicalis KCTC 7212 were 17.8 g/L with YEtOH of 0.48 at 120 h and 19.3 g/L with YEtOH of 0.50 at 120 h, respectively.
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KEYWORD
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Seaweed, Gelidium amansii, Pretreatment, Ethanol fermentation, SHF
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